荧光素酶/荧光素酶(萤火虫)/重组型酵素/Luciferase firefly

英文名称：Luciferase firefly；Luciferase from Photinus pyralis (firefly)

其他名称：荧光素酶(萤火虫)；重组型酵素

级别：BR

product line：BioUltra

Assay：≥98% (SDS-PAGE)

Mol wt：mol wt 120 kDa (two non-identical subunits, each containing four free thiol groups, one of which is necessary for activity)

Protein：10-35% E1%/280

Measuring range：≤1 femtomole ATP sensitivity, (using 0.2 μg of luciferase and suitably sensitive liquid scintillation counters or luminometers)

Foreign activity：ATPase ≤5 nanomolar units/mg protein；Nucleoside diphosphokinase ≤20 nanomolar units/mg protein

活力定义：One light unit produces a biometer peak height equivalent to 0.02 μCi of ¹⁴C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C

产品描述：Two contaminant, ATP-consuming activities are assayed for in this product, ATPase and nucleoside diphosphokinase. These impurities are found to be less than 5 nanomolar units/mg protein and less than 20 nanomolar units/mg protein, respectively.

Note: Prior to 1991, a unit of firefly luciferase activity was defined as that amount which will produce 1.0 nanomole of pyrophosphate per minute at pH 7.7, 25 °C, using a system containing 0.6 mM ATP and 0.1 mM D-luciferin. The former nanomolar unit is equivalent to approximately 1.3 x 10⁶ light units

性状：粉末。EC：1.13.12.7

用途：生化研究。