磷酸二酯酶Ⅱ/磷酸二脂酶/磷酸二酯酶/Phosphodiesterase II from bovine spleen

英文名称：Phosphodiesterase II from bovine spleen；3′-Exonuclease；Spleen exonuclease；Spleen phosphodiesterase

其他名称：磷酸二脂酶；磷酸二酯酶

级别：BR

Activity：≥10u/mg

活力定义：One Unit increases the absorbance at 260 nm by 0.200 in 30 minutes at 37℃, pH 6.5, with an RNA substrate

产品描述：Spleen exonuclease (Phosphodiesterase II) excises 3'-phosphomononucleotides from oligonucleotides having a free OH terminus. The optimum pH for the enzyme is 5.5 using succinate and phosphate buffer and pH 6-7 with 0.1 M acetate buffer. The enzyme is assayed using a modification of the Hilmoe (Biochem. Prep., VII, (Meister, A., ed.), John Wiley and Sons, NY, 105, 1961) technique

性状：粉末

用途：生化研究。