GST琼脂糖凝胶4FF/谷胱甘肽琼脂糖凝胶4FF/Glutathione Sepharose 4FF

英文名称：Glutathione Sepharose 4FF
其他名称：谷胱甘肽琼脂糖凝胶4FF

级别：BR
Ligand：glutathione and 10-carbon linker arm
Ligand concentration：120-320umol glutathione/ml medium
Binding capacity：～10mg recombinant glutathione S-transferase/ml medium,GST Mr26000
Dynamic binding capacity：～11mg GST-tagged protein/ml medium Mr43000(GSTrap FF 1ml at 1 ml/min=156cm/h)
Mean particle size：90um
Bead structure：highly cross-linked 4% agarose
Maximum flow rate：450cm/h(15ml/min),using xk16/20 column with 5cm bed height,run at room temperature with aqueous buffer
Recommended flow rate：Sample loading:＜100cm/h(＜3ml/min using XK16/20 column)；Washing and elution:100～300ml/min using XK16/20 column)
PH stability：3-12
Chemical stability：All commonly used aqueous buffers,e.g. 1M acetate pH 4.0 and 6M guanidine hydrochloride for 1 hour at room temperature
性状：含20%乙醇，底部为乳白色胶体
用途：生化研究。专门用于纯谷胱甘肽S-转移酶（GST）融合蛋白、其他的谷胱甘肽转移酶以及与谷胱甘肽有亲和作用的亲和色谱填料，一步分离就可得到很纯的目标蛋白，纯化条件温和，可保证蛋白的活性