Thiazole Blue / thiazolam / thiazolium bromide tetrazolium / MTT

English name：MTT；Thiazolyl Blue Tetrazolium Bromide；3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide；Thiazolyl blue；Methylthiazolyldiphenyl-tetrazolium bromide

Other names: 3 - (4,5-dimethyl-2-thiazole) - 2,5-diphenyl tetrazolium bromide thiazole blue; Thiazolam; Thiazole blue tetrazolium bromide; 3 - (4,5-dimethyl-2-thiazolyl) - 2,5-diphenyl-2h-tetrazolium bromide; 2 - (4,5-dimethyl-2-thiazolyl) - 3,5-diphenyl-2h-tetrazolium hydrobromide

CAS No.: 298-93-1

C18H16BrN5S=414.32

Grade: br

Content: ≥ 98.0%

Heavy metal: ≤ 5ppm

Moisture: ≤ 0.5%

Physicochemical properties: light yellow or yellow powder or orange yellow powder, slightly soluble in methanol, but difficult to dissolve in water or ethanol. It cannot be completely dissolved with ether, acetone or ethyl acetate. Dissolved in water or DMSO at 195 ℃. It is usually prepared into 5mg / ml stock solution. Melting point: 195 ℃ (decomposition)

Purpose: biochemical research. It is commonly used for cell viability, cell proliferation or cytotoxicity detection based on MTT detection methods. Because MTT has a positive charge, it can pass through the cell membrane and be reduced by mitochondria to form purple methamphetamine dye. Organic solvent is required to dissolve MTT methamphetamine dye. Detection of dehydrogenase, determination of enzyme activity in biochemical studies, determination of NADH2, NADPH2 and diaphorase.

Storage: 2 ~ 8 ℃, protected from light