Nitro tetrazolium chloride Blue / nitro blue tetrazolium / nitro BT / NBT

English name：NBT；Nitroblue tetrazolium chloride；Nitroblue tetrazolium；2,2'-Bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride

Other names: azoblue tetrazole; Nitroblue tetrazolium; Nitro BT; Nitrotetrazolium chloride; Nitrotetrazolium chloride blue; Azotetrazolium blue; Tetrazolium chloride blue

CAS No.: 298-83-9

C40H30Cl2N10O6=817.65

Grade: br

Content: ≥ 98.0%

Methanol dissolution test: acceptable

Absorbance EM (lambda max, methanol): > 54000

Physicochemical properties: light yellow powder or yellow needle crystal. There is no obvious light instability. Easy to absorb moisture. Soluble in water and ethanol, slightly soluble in ethyl acetate, insoluble in ether. Its reductant (diformazan) is a black purple crystalline powder, which is soluble in most organic solvents. Toxic. Carcinogenic possibility and irritation

Use: substrate of dehydrogenase and other oxidase. For the determination of glucose-6-phosphate dehydrogenase. It is also a precipitation reagent of coagulation factor I (fibrinogen). It is also one of the substrates of alkaline phosphatase and generates insoluble Blue products under the catalysis of alkaline phosphatase. It is often used in immunohistochemistry (IHC), immunocytochemistry (ICC), Western blot (WB) and other experiments. Quantification of oxidoreductase and steroids. Determination of succinate dehydrogenase in histochemistry. Determination of clinical chemical isoenzymes. It is used as a cell stain because it can be reduced to a blue violet azo compound (diformazan) by dehydrogenases in malignant cells.

Storage: 2 ~ 8 ℃, protected from light