Methylene blue / methylene blue / methylene blue / methylene blue / methylene blue / magenta Blue

English name：Methylene Blue trihydrate；C.I. 52015 trihydrate；Basic Blue 9 trihydrate； 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride

Other names: methylene blue; Meilan;reddish blue;Alkaline Blue 9; Tetramethyl blue;  Alkaline lake blue BB; Alkaline methylene blue; Salt based lake blue; Basic lake blue BB; Methylene azure; 3,7-bis (dimethylamino) phenothiazine-5-weng chloride; Methylene blue trihydrate;Chloro-3,7-bis (dimethylamino) phenothiainium-5-onium

CAS No.: 7220-79-3

C16H18ClN3S•3H2O=373.90

Grade: BS ind, 82% reagent grade, 98%

Biological staining test: qualified

Ethanol dissolution test: acceptable

Burning residue: ≤ 0.5%

Loss on drying: ≤ 15.0%

Physicochemical properties: shiny dark green crystal or fine dark brown powder, difficult to dissolve in cold water and alcohol, easy to dissolve when heated. With copper luster, odorless, stable in the air. 1g is dissolved in about 25ml of water and about 65ml of ethanol. The solution is sky blue, soluble in chloroform, and insoluble in ether and benzene. The aqueous solution can fade when it meets with zinc powder and dilute sulfuric acid, but it can recover when exposed to air. If ammonia water is added, the recovery will be faster. It can form complex salt with most inorganic salts, and the maximum absorption wavelength is 668 and 609 nm. Low toxicity, half lethal dose (rat, oral) 1180mg / kg. Slightly soluble in alcohol, it is blue; It turns yellow green when encountering concentrated sulfuric acid; Blue after dilution; When sodium hydroxide solution is added to the aqueous solution, it will appear purple or dark purple precipitation. Methylene blue indicator is colorless in reduced state, blue in oxidized state, and sensitive in nature.

Purpose: biochemical research. Bacterial stain; Mixed indicator is used for biochemical test of serum protein; Prepare staining solution for exfoliated cell test; Prepare alkaline methylene blue dyeing solution; Redox indicator; Determination of mercury and tin. A reducing titrant for precipitating perchlorate and rhenate; Spectrophotometric Determination of selenium and molybdenum

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