Red tetrazolium / 2,3,5-triphenyltetrazolium chloride / TTC / tptz / ttz / RT

English name：TTC；TTZ；TPTZ ；RT ；Tetrazolium red；2,3,5-Triphenyltetrazolium chloride；Red tetrazolium；2,3,5-Triphenyl- 2H-tetrazolium chloride；Tetrazolium salt；Tetrazolium chloride；Vitastain

Other names: 2,3,5-triphenyltetrazolium chloride; Chlorotriphenyltetrazolium; Tetrazolium red; Red tetrazole; 2,3,5-triphenyltetrazolium chloride; Triphenyltetrazolium chloride; 2,3,5-triphenyl-2h-tetrazolium chloride; Triphenyltetrazole chloride

CAS No.: 298-96-4

C19H15N4Cl=334.80

Grade: br

Content: ≥ 98.0%

Molar absorption coefficient: ≥ 2 × 104L/cm•mol

Sensitivity test: acceptable

Dissolution test: acceptable

Moisture: ≤ 5.0%

Burning residue: ≤ 0.5%

Add an appropriate amount of TTC to the Counting Agar (0.5% TTC 1ml is added to 100ml agar), and the bacterial colonies grow into red color, which is very meaningful to remove the interference of food background particles. In drug analysis - tetrazolium salt colorimetry:

The types of tetrazolium salts are as follows: ① 2,3,4-triphenyltetrazolium chloride (TTC), also known as red tetrazolium (RT), the reduction product of which is a dark red triphenyl (Zan) hairpin insoluble in water, λ Max at 480 ~ 490nm, ② blue tetrazolium (BT), i.e. 3,3 '- dimethoxyphenyl bis-4,4' - (3,5-diphenyl) tetrazolium chloride, whose reduction product is dark blue dimethylzan hairpin, λ Max is about 525nm.

Reaction principle: The corticosteroid C17-α-ketone group (-CO-CH2OH) is reducible, and can quantitatively reduce tetrazozole salt to colored methylzan in the strong alkaline test solution. The resulting color varies depending on the reagent and condition used, and is mostly red or blue.

Reaction principle: corticosteroid C17- α- Alcohol ketone group (- co-ch2oh) has reducibility and can quantitatively reduce tetrazolium salt to colored methyl Zan in strong alkaline test solution. The generated color depends on the reagents and conditions used, and is mostly red or blue

Determination method: triphenyltetrazolium chloride method was used in Chinese Pharmacopoeia. For example, content determination of prednisolone acetate ointment

Physicochemical properties: light yellow or white powder. It is hygroscopic. The color becomes darker when encountering light. Soluble in water, ethanol and acetone, insoluble in ether. Melting point 243 ℃ (decomposition)

Purpose: biochemical research. A sensitive reagent for reducing sugar. Distinguish ethanol, ketones and simple aldehydes. The activity of dehydrogenase was measured. Titrate diborane, pentaborane and decaborane. Pesticide residue analysis.

Storage: 2 ~ 8 ℃, protected from light