Hot Taq DNA Polymerase
Item No.： E0086-1
Store at： -20℃
English name ：Hot Taq DNA Polymerase
Other name: Hot Taq DNA polymerase
Grade：BR
Purity：≥99%
Concentration：5u/ul
Activity definition: the amount of enzyme required to infiltrate 10nmol dNTPs into acid insoluble substances at 75 ℃ for 30 minutes.
Product composition：Hot Taq DNA Polymerase，10×PCR Buffer，MgCL2
Physicochemical properties: Liquid.Hot Taq DNA Polymerase is chemically modified Taq DNA polymerase.It is suitable for hot start PCR. Because it is chemically modified, it inhibits the activity of Taq enzyme. At room temperature, the enzyme activity is blocked and heated at 94 ℃ for 10 minutes to return to normal activity and start the reaction. DNA can be polymerized. This method inhibits the nonspecific amplification caused by primer nonspecific annealing or primer dimer at low temperature.
Purpose: biochemical research. PCR amplification of complex template DNA; PCR amplification with complex cDNA as template, such as RT-PCR; Sequencing of single or double stranded DNA; Site directed mutation;Real-time PCR.
