Taq DNA Polymerase CAS 9012-90-2
Item No.： E0086
Store at： -20℃
English name：Taq DNA Polymerase
Other name: Taq DNA polymerase
Grade：BR
Purity：≥99%
Concentration：2～5u/ul
Vitality definition：The activity of 1 unit (U) Taq DNA polymerase is defined as the amount of enzyme required for the incorporation of 10mmol deoxynucleotides into acid insoluble substances with the activated salmon sperm DNA as a template / primer within 30 minutes at 74 ℃.
Physicochemical properties: Liquid, is an efficient PCR DNA polymerase. It is isolated and purified from Escherichia coli cloned with thermoaquaticus DNA polymerase gene after induced expression, and its molecular weight is 94KD,Taq DNA polymerase has 5 '- 3' DNA polymerase activity and 5 '- 3' exonuclease activity, but no 3 '- 5' exonuclease activity. In PCR reaction, the extension rate of Taq DNA polymerase is 1-2kb / min, and the product has 3 'end band a, which can be cloned directly with TA vector.
Purpose: Biochemical research. It is generally used for PCR amplification of DNA fragments, DNA labeling, primer extension, sequence determination, flat end plus a, etc; The product can be directly used for t / a vector cloning.
