β-Amylase from Bacilus subtilis；1,4-α-D-Glucan maltohydrolase CAS 9000-91-3
Item No.： E0005
Store at： 2～8°C
English Name: β- Amylase from Bacilus subtilis； 1,4- α- D-Glucan maltohydrolase
Other names: 1,4- α- D-glucan malt hydrolase; Glycogen enzyme; Glucoamylase
Grade: br
Activity: ≥ 50U / mg
Grade: high purity
Activity: ≥ 100u / mg
Definition of enzyme activity unit: under the conditions of 60 ℃ and ph5.6, the amount of enzyme releasing 1 mmol of maltose from 2% soluble starch solution per hour is defined as 1 enzyme activity unit (U)
Physicochemical properties : brown powder. Bacillus subtilis extract, soluble in water and dilute buffer, almost insoluble in ethanol. Enzyme reaction: starch (soluble) + nH2O = n maltose + dextrin. The optimum pH is 4.0 ~ 6.0. The optimum temperature is 50 ~ 60 ℃. Isoelectric point (PI) 4.77. Light absorption coefficient (E1% 280) 12.3. Inhibitors include cyclohexane amylose and methyl- α- Glucoside, sulfhydryl reagent (4-chloromercuric benzoic acid, iodoacetamide), heavy metal ions (Cu2 +, HG2)
Purpose: biochemical research. It can decompose amylose into maltose. The production of Isomaltooligosaccharides (imo50, imo90) with transglycidase is better than fungal enzyme. Super high malt can be produced by combining with pullulanase.
