Carboxypeptidase Y(Yeast);Carboxypeptidase Y from baker's yeast CAS 9046-67-7
Item No.： E0135
Store at： -20℃
English name：Carboxypeptidase Y from baker's yeast
CAS NO：9046-67-7
Grade：BR
Vitality：≥50 units/mg protein
Vitality definition：One unit will hydrolyze 1.0 μmole of N-CBZ-Phe-Ala to N-CBZ-L-phenylalanine and L-alanine per min at pH 6.75 at 25°C, based on EM/230=191.5
Product description：A glycoprotein exopeptidase of the “acid” and “serine” calss.Highly purified specific activity in two different substrate systems：Z-Phe-Leu，Z-Glu-Tyr.
Physicochemical properties : White powder or solid, isoelectric point 3.6, extinction coefficient a at 280 nm is 15.0.The enzyme can be inhibited by diisopropyl fluorophosphate (DFP),It can also be inhibited by some metal ions such as Cu2+、Hg2+、Fe2+、Mg2+, etc.
Purpose: Biochemical research.A class of Exopeptidases that can specifically degrade one by one from the C-end of the peptide chain and release free amino acids.It exists in organisms in the form of zymogen.Carboxypeptidase Y has extensive hydrolysis ability to various amino acids at the carboxyl end, so it has become a common tool enzyme in the C-terminal analysis of protein polypeptide chain. Unlike pancreatic carboxypeptidase A and B, it is an acidic protein without metal ions and has peptidase and lipase activities.
