BCHE；BuCHE ；Cholinesterase,butyryl(Horse serum) ；Acylcholine acyl-hydrolase CAS:9001-08-5
Item No.： E0136
Store at： 2～8°C
English name：BCHE；BuCHE ；Cholinesterase,butyryl(Horse serum) ；Acylcholine acyl-hydrolase；Choline esterase, butyryl；Pseudocholinesterase
Molecular weight：～440000
Grade：BR
Vitality：≥4units/mg
Product description：It catalyzes the hydrolysis of a number of choline esters according to the following reaction：Acylcholine+H2O to choline+acid.It is a tetrameric glycoprotein with four subunits of equal size,each having a molecular weight of 110KDa.Butyryl cholinesterase hydrolyzes butyrylcholine four times more rapidly than acetylcholine.It is inhibited by 10-5 M physostigmine，numerous organophosphate esters,the carbamate derivates and quaternary ammonium saltsunit hydrolyzes 1umole of acetylchoine per minute at 25℃, pH7.4
Physicochemical properties: Light brown powder.There are two kinds of cholinesterase: ache and butyryl cholinesterase (BuChE).The gene structure, amino acid sequence and spatial three-dimensional structure of both showed high homology.The main difference between the two is that ache hydrolyzes acetylcholine (ACh) and acetylthiocholine (atch) faster than propionylcholine (PRCH), and has no obvious hydrolysis effect on butyrylcholine (Buch) and butyrylthicholine (Butch).The color reaction principle of butyrylcholinesterase and acetylcholinesterase is exactly the same.Butyrylcholinesterase has the advantage of higher sensitivity.
Purpose: Biochemical research.It is a glycoprotein that hydrolyzes choline acetate.It is not only a key enzyme in biological nerve conduction, but also a target of organophosphorus and carbamate insecticides.This enzyme is a powerful antidote against nerve gas bombs. It can neutralize organic phosphate containing nerve agents, pesticides and chemical fluxes, so as not to be disturbed by nerve agents.
