PK；Pyruvate Kinase from microorganism；ATP:pyruvate 2-O-phosphotransferase CAS:9001-59-6
Item No.： E0146
Store at： -20℃
English name：PK；Pyruvate Kinase from microorganism；ATP:pyruvate 2-O-phosphotransferase
Other names: Pyruvate phosphotransferase; Phosphopyruvate kinase
Molecular weight：68KDa
Grade：BR
Vitality：≥300units/mg protein
Vitality definition：1 U will convert one micromole of phosphoenolpyruvate to pyruvate per minute at pH 7.2 and 30 °C
pH stability：5.0～10.0(37℃,20hr)
Optimum PH：7.5
Thermal stability：≤60℃(pH8.5, 20min)
Optimum temperature：80°C
Preparation instructions：The enzyme is reconstituted in 50mM Tris-HC1 buffer,pH8.5 for activity assay
Physicochemical properties: White powder, composed of 4 subunits (molecular weight 5500).Long chain fatty acids, acetyl CoA, ATP and alanine inhibited the enzyme activity, and fructose 1,6-diphosphate activated it.
Purpose: Biochemical research.Pyruvate kinase, a kinase involved in the reaction of step 10 of glycolysis pathway, is an individual constitutive enzyme.It catalyzes the conversion of phosphoenolpyruvate and phosphorylates ADP to ATP.
