UAO；Uricase；Urate oxidase CAS:9002-12-4
Item No.： E0148
Store at： -20℃
English name：UAO；Uricase；Urate oxidase
Other names: Urease; Uric acid oxidase
Molecular weight：34KDa(SDS-PAGE)
Grade：BioChemika
Vitality：≥10unit/mg protein
Activity definition: the amount of enzyme required to oxidize 1umol uric acid per minute at pH 8.5 and 25 ° C
Isoelectric point PI：5.4
PH stability：7.0～11.0
Optimum pH：8.5
Thermal stability：＜50℃（PH8.5，10min ）
Optimum temperature：45℃
Usage: when measuring the activity, dissolve the lyophilized powder with 50 mm boric acid buffer (ph8.5) containing 0.001% Triton X-100 and 1 mM EDTA
Physicochemical properties: Extracted from fermentation broth of Aspergillus niger, Aspergillus flavus and Candida.White lyophilized powder or gray white to brownish green crystal or bright transparent sheet.Almost insoluble in water, slightly soluble in alkaline buffer.The enzyme activity is inhibited by cyanide, such as Ag+，Hg2+
Purpose: Biochemical research.It can catalyze the rapid oxidation of uric acid and finally produce allantoic acid. It is used to determine the amount of urinary acid in serum and urine.
