Urokinase;Urokinase from human urine CAS:9039-53-6
Item No.： E0149
Store at： -20℃
English name：Urokinase from human urine
Other names: Yakinase; Urinary activin; U-Plasminogen activator
Grade：BR
Vitality：≥120000IU/mg
Titer ： ≥50000IU/mg
Vitality definition：37℃，pH8.8，Amount of enzyme required for hydrolysis per minute required for hydrolysis of 1μmole Be-Ala-Gly-Arg-4Na is one unit.
Solubility: Complete dissolution within 60 seconds(3000IU/ml)
High molecular content：≥85% (M.W.54000)
Pyrogen：Compliance with regulations（20000IU/ml.Kg.）
Endotoxin：≤2 EU/20000IU
Abnormal toxicity：Negative (2500IU/0.5 ml/mice)
HBs-Ag：Negative (50000 IU/ml)
Anti-HIV I + II：Negative(50000 IU/ml)
nti-HCV Abs：Negative (50000 IU/ml)
Physicochemical properties: White or off white freeze-dried powder.It is a proteolytic enzyme of human urine or renal cells.The natural substrate is plasminogen.Sensitive to moisture.Soluble in water.The dry powder is stable at 4 ℃, and the stable period of aqueous solution at 4 ℃ is 3 days.
Purpose: Biochemical research.It is a protease that cleaves fibrinogen to form plasmin.It binds to cell surface receptor (uPAR), regulates cell adhesion, tissue remodeling and migration, and activates intracellular signal transduction pathway.Urokinase is composed of three domains: the amino terminal domain homologous to epidermal growth factor and the protease domain bound to the carboxyl terminal through the central kringle domain.
