HSD；3α-Hydroxysteroid Dehydrogenase from microorganism CAS:9028-56-2
Item No.： E0151
Store at： -20℃
English name：HSD；3α-Hydroxysteroid Dehydrogenase from microorganism；3α-Hydroxysteroid:NAD(P)+ oxidoreductase
Other names:Hydroxyl steroid oxidoreductase; oxysterols
CAS NO：9028-56-2
Grade：BR
Molecular weight：28KDa(SDS-PAGE)
Vitality：≥50units/mg protein
Vitality definition：One unit will oxidize one micromole androsterone per min at pH 8.9 at 25 °C
Protein：≥25%
Isoelectic point：7.5
PH stability：4.5～10.5
Optimum pH：10.5
Optimum temperature：60℃
Thermal stability：＜50℃(pH7.2，20min)
Inhibitor：Ag+，Hg2+
Preparation instructions：The enzyme is reconstituted in 30mM potassium phosphate buffer,PH7.2 for activity assay.
Physicochemical properties: White powder
Purpose: Biochemical research.It catalyzes the dehydrogenation of hydroxyl steroids and is used for the clinical detection of total bile acids.
