Neutral protease/Dispase CAS 9068-59-1
Item No.： E0011
Store at： 2～8°C
English Name: Neutral protein; Dispase
Other name: Dispersing enzyme
Grade: BR
Vitality: ≥ 50units/mg
Grade: High purity
Vitality: ≥ 100units/m
Definition of activity: 1mg solid enzyme powder hydrolyzes casein at 30 OC and ph7.5 for 1 minute to produce 1ug tyrosine as an enzyme activity unit, expressed in U/mg
Stable pH: 5.5 ~ 8.5
Optimum pH: 6.8 ~ 7.0
Optimum temperature: 45 ~ 50 ℃
Physicochemical properties : yellowish brown powder, which is a metabolite produced by Bacillus subtilis after submerged fermentation. It is made into powder by salting out, drying and grinding. It is easy to dissolve in water. Most neutral proteases produced by microorganisms are metalloproteinases. One molecule of protease contains one atom of zinc, with a molecular weight of 35000 ~ 40000 and an isoelectric point of 8 ~ 9. It is the most unstable enzyme in microbial protease. It is easy to autodissolve and will cause a significant reduction in molecular weight
Application: biochemical research, extraction of polysaccharides and hydrolysis of various plant proteins. Under neutral conditions, it catalyzes the decomposition of proteins or high molecular peptide bonds, and its products are small molecular peptones, small peptides and individual amino acids.
