Lipoxidase from soybean CAS:9029-60-1
Item No.： E0168
Store at： 2～8°C
English name：Lipoxidase from soybean；Linoleate:oxygen oxidoreductase；Lipoxygenase
molecular weight：108 kDa (two 54 kDa subunits)
Grade：BR
Vitality：≥50000units/mg protein
Vitality definition：One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid
Physicochemical properties: powder or suspension. It exists in legumes such as alfalfa, pea, lentil and potato, but the activity is the strongest in soybean. The isoelectric point of lipoxygenase crystallized from soybean is 5.4. The optimum pH cannot be simply determined, but is determined by the solubility of the substrate, generally ph6 0 ~ 3.0, and the optimum temperature is 20 ~ 30 ℃.
Purpose: biochemical research. Lipoxygenase can catalyze molecular oxygen to oxidize oil with glutaric 1,4 double bond to form hydroperoxide. Hydroperoxide oxidizes the sulfhydryl (- SH) of protein molecules to form disulfide bonds (- S-S -) and can induce protein molecules to polymerize, making protein molecules larger. Lipoxygenase can destroy the double bond structure of carrot through coupling reaction, so as to bleach and improve color
