Triosephosphate Isomerase；D-Glyceraldehyde-3-phosphate ketol-isomerase CAS:9023-78-3
Item No.： E0169
Store at： -20℃
product name：TPI；TIM； Triosephosphate Isomerase；D-Glyceraldehyde-3-phosphate ketol-isomerase
Grade：BR
Vitality：≥3500 units/mg protein
Vitality definition：One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C
Pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase and glyceraldehyde-3-phosphate dehydrogenase ：<0.01%
Protein：70% biuret
Physicochemical properties: Powder.Sulfate-free, contains EDTA and borate buffer salts。
A dimer formed by two identical subunits; Each subunit contains about 250 amino acid residues. The three-dimensional structure of each subunit contains 8 external subunits α The helix is parallel to the 8 internal β Chain. Such a structural pattern is called αβ Barrel or TIM barrel is the most common protein folding mode observed at present. The active site of the enzyme is located in the center of the "barrel", in which a glutamate and a histidine participate in the catalytic reaction process. The sequence of residues near the active site is conserved in all known triose phosphate isomerases
Purpose: biochemical research. It can catalyze the conversion of propyl phosphate isomers between dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate. Triose phosphate isomerase plays an important role in glycolysis and is essential for effective energy generation
