Phosphodiesterase I from Crotalus adamanteus venom CAS:9025-82-5
Item No.： E0194
Store at： -20℃
English Name: phosphodiesterase I from Crotalus adamanteus venom; 5′-Exonuclease； Oligonucleate 5′-nucleotidohydrolase
Other names: phosphodiesterase; phosphodiesterase
Grade: BR
Activity：≥20u/mg
Vitality definition：One Unit hydrolyzes one micromole of p-nitrophenyl thymidine-5-phosphate per minute at 25℃, pH 8.9.
Product description：Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-OH-terminated ribo- and deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg2+
Physicochemical properties : white or off white powder
Purpose: biochemical research.
