Phosphodiesterase II from bovine spleen CAS:9068-54-6
Item No.： E0195
Store at： -20℃
product name：Phosphodiesterase II from bovine spleen；3′-Exonuclease；Spleen exonuclease；Spleen phosphodiesterase
Other names: phosphodiesterase; phosphodiesterase
Grade：BR
Activity：≥10u/mg
Vitality definition：One Unit increases the absorbance at 260 nm by 0.200 in 30 minutes at 37℃, pH 6.5, with an RNA substrate
Product description：Spleen exonuclease (Phosphodiesterase II) excises 3'-phosphomononucleotides from oligonucleotides having a free OH terminus. The optimum pH for the enzyme is 5.5 using succinate and phosphate buffer and pH 6-7 with 0.1 M acetate buffer. The enzyme is assayed using a modification of the Hilmoe (Biochem. Prep., VII, (Meister, A., ed.), John Wiley and Sons, NY, 105, 1961) technique
Physicochemical properties : Powder
Purpose: biochemical research.
