EcoRI endonuclease
CAS No.:
Item No.: E0228
Store at: -20℃
Item No.: E0228
Store at: -20℃
Overview: this restriction enzyme recognizes the cleavage site of 5 '- G ↑ aattc-3' and has the best activity in EcoRI buffer.
Features: all endonucleases have 100% activity in the optimal buffer and unified buffer; The buffer is 100% compatible with downstream experimental applications.
Application: enzyme digestion
Instructions for use: high enzyme concentration and the use of non optimal buffer may lead to star activity. Do not incubate for a long time when using BSA. In order to obtain 100% activity, BSA should be added to the 1x reaction mixture to a final concentration of 100 μ g/ ml.