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HK;Hexokinase from Saccharomyces cerevisiae CAS 9001-51-8

CAS No.: 9001-51-8
Item No.: E0019
Store at: -20℃

Other name: Hexose phosphate kinase

Grade: BR

Molecular weight:~ 54 kDa (monomer);~110 kDa (dimer)
Vitality:~ 500 units/mg protein
Vitality definition:One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25℃ 
Optimal pH:7.5~9.0
Physicochemical properties (the following information is for reference only): white powder. Saccharomyces cerevisiae extract.Catalyzer(Activators):Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds;Inhibitor(Inhibitors):sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
Purpose: This product is only for scientific research and shall not be used for other purposes. (the following purpose is for reference only) it is an enzyme that catalyzes the phosphorylation of hexose by ATP at position 6. yes α- D has high affinity for glucose (km = 1) × 10-5m), and other hexose can also be phosphorylated. The balance of this reaction shifts to the right. △ g ° '= 4.0 kcal. The enzyme can be activated by Mg2 and K, but can be inhibited by HS reagent.
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