DEAE Sephadex A-25 CAS:12609-80-2
Item No.: I0044
Store at: RT
English name:DEAE Sephadex A-25
Other names: two ethyl amino ethyl cross-linked dextran gel A-25; Dextran gel DEAE-A25
Type of ion exchanger: Weak anion
Total ionic capacity(mmol/g dry powder):3~4
Available capacity(mg/ml avained gel):
①thyroglobulin(MW669000):1
②HSA(MW68000):30
③α-lactalbumin(MW14300):140
Bead structure:Cross-linked dextran
Dry particle size range:40~120um
Max operating linear flow rate:475cm/h at 25℃;HR 16/10 column;5cm bed height
pH working range:2~9
pH stability:long term and short term 2~13
Chemical stability:Stable to all commonly used aqueous buffers and additives such as 8 M urea and 6 M guanidine hydrochloride
Physical stability:Negligible volume variation due to changes in pH or ionic strength
Physicochemical properties: Anion exchanger.
Purpose: Biochemical research.Low molecular weight proteins, peptides, nucleotides and giant molecules(MW>200KD)