Pancreatin；Pancreatin from hog pancreas CAS 8049-47-6

English name：Pancreatin；Pancreatin from hog pancreas
Other names: Trypsin; Pancreatic enzyme; Trypsin; Pancreatin; Trypsin; Pancreatic enzyme

Grade: BR

Molecular weight: about 23400

Potency: 1:4000

Trypsin activity: ≥ 4000U/g

Pancreatic amylase activity: ≥ 30000u/g

Pancreatic lipase activity: ≥ 7000u/g

Loss on drying: ≤ 5.0%

Fat: ≤ 20mg/g

Dissolution method 1: dissolve pancreatin with 90ml of Ca and Mg free PBS, and then add the PBS to 100ml (the enzyme is difficult to dissolve, and it can be dissolved overnight at 4 degrees if necessary). Adjust pH by about 7.2 and 5.6% NaCO3.

Dissolution method 2: maintain the temperature of the solution in the reaction pot at 80 ℃ for at least half an hour, and then feed tap water from the interlayer to reduce the solution temperature in the pot to 50 ℃, add the required amount of trypsin according to the enzyme / substrate ratio of 1 ∶ 30, and start the enzymatic hydrolysis reaction under the continuous stirring of the agitator. During this period, adjust the amount of steam fed into the interlayer of the reaction pot to maintain the temperature at 50 ℃. In the process of enzymatic hydrolysis, in order to ensure the maximum biological activity of trypsin and maintain the pH of the reaction solution at 8.0, it is necessary to continuously add NaOH dilute solution to the reaction solution. The enzymatic hydrolysis reaction ended 6 hours after the addition of trypsin

Dissolution method 3: accurately weigh 8.00g NaCl, 0.40g KCl, 0.09g Na2HPO4 · 7H2O, 0.06g KH2PO4, 0.01g phenol red and 0.35g NaHCO3, dissolve them in about 800ml five distilled water, fully dissolve them, fix the volume to 1000ml, repack them, sterilize them for standby, and prepare d-hanks solution. Accurately weigh 0.125g trypsin, dissolve it in 100ml d-hanks solution, overnight at 4 ℃, filter and repack it, and store it at - 20 ℃ for standby.

Physicochemical properties : off white or light yellow amorphous powder. It is a mixture of protease, lipase and amylase prepared from various animal pancreas. Hygroscopic. Insoluble in ethanol and ether. The enzyme activity is lost when the aqueous solution is boiled or in the presence of acid. Its maximum activity is in neutral or slightly alkaline medium. Mineral acid or excess hydroxide or carbonate deactivates it.

Purpose: biochemical research. In neutral or weakly alkaline environment (pH 6.8 ~ 8.5), trypsin can convert protein into peptone, amylase can convert starch into sugar, and pancreatic lipase can decompose fat into glycerol and fatty acids.