Glutathione Sepharose 4FF

English name：Glutathione Sepharose 4FF
Other name: glutathione agarose gel 4FF

Grade：BR
Ligand：glutathione and 10-carbon linker arm
Ligand concentration：120-320umol glutathione/ml medium
Binding capacity：～10mg recombinant glutathione S-transferase/ml medium,GST Mr26000
Dynamic binding capacity：～11mg GST-tagged protein/ml medium Mr43000(GSTrap FF 1ml at 1 ml/min=156cm/h)
Mean particle size：90um
Bead structure：highly cross-linked 4% agarose
Maximum flow rate：450cm/h(15ml/min),using xk16/20 column with 5cm bed height,run at room temperature with aqueous buffer
Recommended flow rate：Sample loading:＜100cm/h(＜3ml/min using XK16/20 column)；Washing and elution:100～300ml/min using XK16/20 column)
PH stability：3-12
Chemical stability：All commonly used aqueous buffers,e.g. 1M acetate pH 4.0 and 6M guanidine hydrochloride for 1 hour at room temperature
Physicochemical properties: 20% ethanol, milky white colloid at the bottom

Purpose: Biochemical research.It is specially used for pure glutathione S-transferase (GST) fusion protein, other glutathione transferases and affinity chromatography fillers that have affinity with glutathione. A very pure target protein can be obtained in one step. The purification conditions are mild and can ensure the activity of the protein.