Sepharose 4FF

English name：Sepharose 4FF

Grade：BR
Matrix: 4% cross-linked agarose

Exclusion limit：60000~20×106 (Globulin)

Shape: spherical

Particle size：45~165 μm
Maximum flow rate：250cm/h
Withstand voltage：0.3MPa
Heat-resisting：120℃,pH7,20min in water

PH applicable range: 2 ~ 14 (short time, on-site cleaning)，2~12(long-term)

Chemical stability: stable at 40 ℃ in the following solutions;2mol/L NaOH;70%EtOH;30% isopropanol；30% acetonitrile；1%SDS;6mol/L  Guanidine hydrochloride；8mol/L  urea

Physicochemical properties: 20% ethanol, milky white colloid at the bottom.Highly coupled agarose gel greatly enhanced mechanical properties and fast flow rate.After enucleation, the nonspecific adsorption is very low and the sample recovery is high;With high chemical stability, it can be operated with a variety of promoting solvents and organic solvents and cleaned in place with 1-2M NaOH.

Purpose: Biochemical research.Isolation of giant molecular viruses and vaccines.