Ni Sepharose 6 Fast Flow

English name：Ni Sepharose 6 Fast Flow
Other name: Metal nickel chelated agarose gel 6FF

Grade：BR
Matrix：Highly cross-linked spherical agarose 6%
Dynamic binding capacity：Approx 40mg(histidine)6-tagged protein/ml medium
Metal ion capacity：Approx 15umol Ni2+/ml medium
Average particle size：90um
Max linear flow rate：600cm/h(20ml/min)using XK16/20 column with 5 cm bed height
Recommended flow rate：＜150cm/h
Max operating pressure：0.1 Mpa,1bar(when packed in XK columns.May vary if used in other columns)
PH stability：long range (≤1 week)3-12 and short range(≤2 hours) 2-14
Chemical stability：Stable in 0.01M HCL,0.1M NaOH,Tested for 1 week at 40℃；1 M NaOH,70% acetic acid.Tested for 12 hours,2% SDS,Tested for 1 hour. 30% 2-propanol .Tested fo 30 min
Physicochemical properties: 20% ethanol, colloid at the bottom.The principle is that some amino acids on the protein surface, such as histidine, can interact with a variety of transition metal ions such as Cu2+，Zn2+，Ni2+，Co2+，Fe3+，It can adsorb proteins rich in these amino acids, so as to achieve the purpose of separation and purification.Therefore, agarose gel coupled with these metal ions can selectively isolate these proteins containing multiple histidine and peptides, proteins and nucleotides that adsorb metal ions.Cysteine and tryptophan can also bind to fixed metal ions, but this binding force is much less than that of histidine residues to metal ions.It has the advantages of good specificity and fast flow rate, uniform particle size, small particle size, more stable chelated nickel, tolerance to higher reducing agents, and good physical and chemical stability.

Purpose: Biochemical research.The recombinant protein with his tag and the polypeptide, protein, nucleotide and phosphorylated protein that can be adsorbed by metal ions were isolated.