ProteinA SepharoseCL-4B

English name：ProteinA SepharoseCL-4B
Grade：BR
Ligand density：～3mg protein A/ml drained medium
Available capacity：～20mg human IgG/ml drained medium 
Bead structure：4% cross-linked agarose
Bead size range：45～165um
Mean bead size：90um
Max linear flow rate：25℃，150cm/hr；HR16/10 column，5cm bed height
PH stability：High and long range 3 ~ 9；Short range (cleaning, regeneration, disinfection)2～10
Note: Protein A is hydrogenated at low pH, and the functional relationship between protein A and pH is unknown
Chemical stability: stable in frequently used buffer（6M guanidine hydrochloride, 8M urea）
Physical stability: There is a weak volume change with the change of pH or ionic strength.
Cleanse：2%Hibitane/20% ethanol/70%ethanol
Physicochemical properties(the following information is for reference only): It contains 20% ethanol and the bottom is colloid.
Purpose: This product is only for scientific research and shall not be used for other purposes.(the following uses are for reference only) it has been proved from some mammalian species that protein A can connect IgG, IgM or IgA,Protein A agarose gel CL-4B has become a powerful and effective tool for the separation and purification of some classes, subclasses and fragments of immunoglobulin in biological culture medium and cell culture medium.Because only the Fe fragment is involved in the connection, the Fab fragment can connect the antigen. Therefore, protein A agarose gel CL-4B is very useful in isolating immune complexes.