Gram Stain Solution(Ⅰ、Ⅱ、Ⅲ)

Inspection principle:

After crystal violet primary dyeing and iodine solution mordant dyeing, a water-insoluble crystal violet and iodine complex is formed in the cell wall. Due to its thick cell wall, many layers of peptidoglycan nets and dense cross-linking, when it is treated with ethanol or acetone decolorization, the mesh will shrink due to water loss. In addition, it does not contain lipids, so there will be no cracks in the ethanol treatment. Therefore, the crystal violet and iodine complex can be firmly left in the wall, Make it still purple; Due to its thin cell wall, high lipid content in the outer membrane layer, thin peptidoglycan layer and poor crosslinking degree, the outer membrane dominated by lipids dissolves rapidly in the presence of decolorizer. The thin and loose peptidoglycan network cannot prevent the dissolution of crystal violet and iodine complex. Therefore, the Gram-negative bacteria are still colorless after decolorization with ethanol, and then re stained with red dyes such as sand yellow, making the Gram-negative bacteria red.

Main components: kit components main components

A. Fuchsin dye fuchsin, ethanol

B: Iodine solution polyvinylpyrrolidone iodine, potassium iodide

C: Crystal violet dye, crystal violet, isopropanol, ethanol

Product usage:

1. Manual dyeing method

① Make a thin smear and fix the flame after drying;

② Drip liquid C onto the fixed smear, preferably cover the smear, dye for 30 seconds, and wash with water;

③ Drip B solution, preferably cover the smear, dye for 30 seconds, and wash with water;

④ Drip liquid a, preferably cover the smear, re dye for 30 seconds, and wash with water;

⑤ After being sucked or dried in the air, put it under the oil microscope for observation.

2. Automatic machine dyeing method

Usage: This product is used for Gram staining of specimens and bacterial smears

Save: RT