Cellulose acetate layer sheets

English name：Cellulose acetate layer sheets

Other names: cellulose acetate membrane; Cellulose acetate membrane; Acetate membrane

Specifications：8×12cm/sheet

                          2×8cm/sheet

Physicochemical properties: White film, made of cellulose acetate.A medium for electrophoretic analysis.Dry brittle.As an electrophoretic support, cellulose acetate film has the following advantages:①The band boundary was clear after electrophoresis;②Short power on time (20 minutes to 1 hour)；③It hardly adsorbs all kinds of proteins (including serum albumin, lysozyme and ribonuclease), so there is no tailing phenomenon;④There is no adsorption on the dye, so the unconjugated dye can be completely washed off, and the place without sample is almost completely colorless. Although its electroosmosis effect is high, it is very uniform and does not affect the separation effect of samples. Due to the low water absorption of cellulose acetate film, electrophoresis must be carried out in a closed container and low current should be used to avoid evaporation.After electrophoretic dyeing of cellulose acetate film, the zone can be cut off and dissolved in a certain solvent for optical density determination.It can also be immersed in oil with refractive index of 1.474 or other transparent liquid to make it transparent, and then measured directly with an optical densitometer.Its disadvantage is that the thickness is small, the amount of sample is very small, and it is not suitable for preparation.

Purpose: Biochemical research.Cellulose acetate membrane electrophoresis has been widely used in the separation and analysis of serum proteins, hemoglobin, globulin, lipoprotein, glycoprotein, alpha fetoprotein, steroids and isoenzymes. Although its resolution is lower than that of polyacrylamide gel electrophoresis, it has the advantages of simplicity and rapidness.According to the physical and chemical properties of the sample, the type, pH and ionic strength of the buffer were selected to improve the electrophoresis speed and resolution.The selected buffer should be highly volatile and have no effect on the observation zone such as color development or ultraviolet light. If the salt content of the sample is high, the salt containing buffer should be used.For example, pH 8 can be selected for serum protein electrophoresis 6 barbital buffer or boric acid buffer;The separation of amino acids can be pH7 2 phosphate buffer, etc.When electrophoresis, first cut the filter membrane into strips of paper with a certain length and width.Mark the position where you want to point the sample with a pencil, point the sample, electrophoresis for a certain time under a certain voltage and current, remove the filter membrane and dye it.Different substances need different color development methods. For example, nucleotides and other substances can be observed and located under the UV analysis lamp, but many substances must be colored by dye.