SP- agarose gel HP/SP Sepharose HP

Product Name: SP agarose gel HP

Corresponding imported product: SP Sepharose HP

Physicochemical properties: white microsphere, gelatinous, preserved in 20% alcohol solution.

Gel type: ion exchange packing, strong acid type.

Structural composition: 6% cross-linked agarose

Particle size: 10-45 μ m

Working pH value: 4-13

Operating temperature: 4-40 ℃

Total ion exchange capacity: 0.12-0.16mmol/ml

Application: it is used for fine purification of protein and other biomolecules by ion exchange.

Purpose: SP- agarose gel HP is a strong cation exchange medium formed by sulfonyl propyl on high flow agarose gel. It is widely used in ion exchange preparation of downstream proteins, nucleic acids and peptides in biopharmaceutical and bioengineering

Preservation: the product shall be stored in a sealed place at 4 ℃ ~ 25 ℃ (the preservation solution is 20% ethanol), ventilated, dry and clean, and shall not be frozen. The used column is stored at 4 ℃ (20% ethanol).

matters needing attention:

(1) Before loading the sample, the sample must be filtered through the membrane and the pigment must be removed, otherwise the impurities and pigment will be adsorbed on the filler and affect the normal use of the filler. All buffers need to be filtered with a 0.45um filter.

(2) In the process of use, avoid using strong acids and bases with high concentration, and the concentration of acids and bases shall be less than 0.1 mol. Alkali slows the flow rate.

(3) Different samples have different adsorption and elution methods, which can be carried out according to the relevant literature.

(4) When selecting chromatographic column for ion exchange medium, avoid using slender column, which will increase the experimental operation pressure