Ni agarose gel 6FF/Ni Sepharose 6FF

Product Name: nickel agarose gel 6FF

Corresponding imported product: Ni Sepharose fast flow

Origin: Domestic

Properties: gelatinous, preserved in 20% alcohol solution.

Gel type: small ligand affinity chromatography packing

Structural composition: 6% cross-linked agarose

Particle size: 45-165um

Ligand group: Ni2+ 

Ligand density: 10-15 μ mol/ml 

Adsorption capacity: 15mg protein / ml

Working pH value: 3-10

Operating temperature: 4-40 ℃

Application: separation of recombinant proteins with his tag and peptides, proteins, nucleotides and phosphorylated proteins that can be adsorbed by metal ions.

Application: metal chelating affinity chromatography medium, also known as fixed metal ion affinity chromatography, its principle is to use some amino acids on the protein surface, such as histidine, to have special interaction with a variety of transition metal ions, such as Cu 2 +, Zn 2 +, Ni 2 +, CO 2 +, Fe 3 +, and can adsorb proteins rich in such amino acids, so as to achieve the purpose of separation and purification. Therefore, agarose gel coupled with these metal ions can selectively isolate these proteins containing multiple histidine and peptides, proteins and nucleotides that adsorb metal ions. In general, Ni2+ is a preferred metal ion for purifying histidine labeled proteins. Cysteine and tryptophan can also bind to fixed metal ions, but this binding force is much less than that of histidine residues to metal ions. Nickel affinity chromatography medium has the advantages of good specificity and fast flow rate, which is very suitable for scale-up production.

Preservation: the used filler shall be thoroughly washed with pure water, and finally stored in 20% ethanol at 4 ℃.

matters needing attention:

1. Before loading the sample, the sample must be filtered through the membrane and the pigment must be removed, otherwise the impurities and pigment will be adsorbed to the filler, affecting the normal use of the filler.

2. In the process of use, avoid the use of high concentration of strong acids and bases, and the concentration of acids and bases shall be less than 0.1 mol. Alkali slows the flow rate.

3. Different samples have different adsorption and elution methods, which can be carried out according to relevant literature.