Phenyl Sepharose/H.P./Phenyl-Sepharose H.P.

Physicochemical properties: white microsphere, gelatinous, preserved in 20% alcohol solution.

Gel type: hydrophobic chromatography media

Structural composition: 6% cross-linked agarose

Particle size: 22-44um

Working pH value: 2-14 (short time); 3-12 (long time)

Operating temperature: 4-40 ℃

Ligand density: 20 μ mol/mL

Application: hydrophobic chromatography medium, fast flow rate, suitable for the separation and purification of biological macromolecules and vaccines.

Purpose: phenyl agarose gel HP is a hydrophobic medium that can be used to achieve the separation and purification of target products by using phenyl interaction on highly cross-linked agarose gel. It is used for purification and separation of biological macromolecules and hepatitis B vaccine.

Preservation: the product shall be stored in a sealed place at 4 ℃ ~ 25 ℃ (the preservation solution is 20% ethanol), ventilated, dry and clean, and shall not be frozen. The used column is stored at 4 ℃ (20% ethanol).

matters needing attention:

(1) Before loading the sample, the sample must be filtered through the membrane and the pigment must be removed, otherwise the impurities and pigment will be adsorbed on the filler and affect the normal use of the filler. All buffers need to be filtered with a 0.45um filter.

(2) In the process of use, avoid using strong acids and bases with high concentration, and the concentration of acids and bases shall be less than 0.1 mol. Alkali slows the flow rate.

(3) Different samples have different adsorption and elution methods, which can be carried out according to the relevant literature