Ribonulease T1 from Aspergillus oryzae；Rnase T1 CAS 9026-12-4
Item No.： E0045-1
Store at： -20℃
English name：Ribonulease T1 from Aspergillus oryzae；Rnase T1
Grade：BR
Molecular weight: 11.2kDa, monomer
Source: E. coli cells contain the cloned gene rnta from Aspergillus oryzae
Concentration: 1000U / ul
Definition of activity: An activity unit refers to the amount of enzyme required to increase the absorbance value of 260nm wavelength by 1.0 by dissolving and hydrolyzing yeast RNA for 15 minutes at 37 ℃ and pH 7.5
Enzyme activity analysis mixture: 50 mm Tris HCl (ph7.5), 2 mm EDTA, 3 mg / ml yeast RNA
Preservation buffer components: 50 mm Tris HCl (pH7.4) and 50% (V / V) glycerol
Quality control: relevant tests show that there is no contamination of endonuclease and exonuclease and protease. The functional test method is RNA degradation during plasmid DNA purification (synergistic effect with RNase A)
Inhibitors: Metal ions Mg2 +, Ca2 +, Zn2 +, Fe2 +, Cu2 + (the inhibition efficiency of MgCl2 at 100 mM concentration is about 40%; the inhibition efficiency of CaCl2 at 10 mM concentration is about 30%; the inhibition effect of Zn2 +, Fe2 + and Cu2 + is very strong at 1 mm), single nucleotide (2-gmp, 3-gmp, etc.), guanylyl-2-5-guanosine
Inactivation: Thermal inactivation is reversible. It is recommended to remove the enzyme by column purification or phenol / chloroform extraction
Physicochemical properties : Ribonuclease T1 is an endonuclease that specifically degrades single stranded RNA at the G residue site. The enzyme cleaves the phosphodiester bond between 3 '- guanosine residue and adjacent nucleoside 5' - OH group by forming nucleoside 2 ', 3' - cyclic phosphate intermediate. The reaction product is 3 '- GMP terminal oligonucleotide. RNase T1 is active without the involvement of metal ions.
Purpose: Biochemical research. Remove RNA from DNA extracts; RNA sequencing; Ribonuclease protection analysis, synergistic effect with RNase A; Remove RNA from recombinant protein extract; The RNA transcripts synthesized by g-less cassette DNA template were detected.
