Ribonulease H CAS 9050-76-4
Item No.： E0045
Store at： -20℃
English name：Ribonulease H
Grade：BR
Molecular weight：18.4kDa，Monomer
Source: Escherichia coli MRE-600 cells
Concentration：2～5u/ul
Definition of activity: one active unit refers to the amount of enzyme required to catalyze the production of 1nmol acid soluble product at 37 ℃ for 20 minutes
Enzyme activity analysis mixture：20mM Tris-HCL(PH7.8), 40mM KC1, 8mM MgCL2, 1mM DTT, 24uM[3H]-poly(A)poly(dT), 0.03mg/ml BSA, 4%(v/v)glycerol
Preservation buffer composition：25mM HEPES-KOH(PH8.0), 50mM KC1, 0.1mM EDTA, 1mM DTT, 0.1mg/ml BSA, 50%(v/v)glycerol
ten × Reaction buffer：20mM Tris-HCL(PH7.8), 400mM KC1, 80mM MgCL2, 10mM DTT
Quality control: relevant tests show that there is no internal or external deoxyribonuclease and ribonuclease pollution
Physicochemical properties: Recombinase is an endonuclease. It can specifically hydrolyze the RNA phosphodiester bond hybridized to the DNA strand, so it can decompose the RNA strand in the RNA / DNA hybridization system. The enzyme cannot digest single or double stranded DNA. Inhibitors include metal chelates and sh blocking reagents
Purpose: Biochemical research. MRNA was removed before second strand cDNA synthesis; RT PCR and QRT PCR; The RNA template was removed after the first strand cDNA was synthesized; Poly (a) sequence in mRNA was removed by hybridization with oligo (DT); Site specific cleavage RNA; Study on polyadenylation reactants in vitro
Storage: - 20 ℃, if heated at 65 ℃ for 10 minutes, it will be inactivated.
