TOPS sodium salt

English Name: tops sodium salt; Sodium 3-(N-ethyl-3-methylanilino)propanesulfonate； 3-(N-Ethyl-3-methylanilino)propanesulfonic acid sodium salt

Other name: n-ethyl-n - (3-sulfonpropyl) m-toluidine sodium salt

CAS No.: 40567-80-4

C12H18NNaO3S=279.33

Grade: high purity grade

Content: ≥ 98%

Physicochemical  Properties: white crystalline powder. dissolve in water

Purpose: biochemical research. Water soluble reagent for the determination of hydrogen peroxide by enzymatic spectrophotometry. The new Trinder's reagent is a highly water-soluble aniline derivative, which is widely used in diagnostic tests and biochemical tests. It has several advantages over conventional chromogenic reagents in colorimetric determination of hydrogen peroxide activity. The new Trinder's reagent is stable enough to be used in both solution and test pipeline detection system. In the presence of hydrogen peroxide and peroxidase, the new trinder's reagent forms very stable purple or blue dyes during the oxidative coupling reaction with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazole sulfone hydrazone (MBTH). The molar absorbance of the dye coupled with MBTH is 1.5-2 times higher than that of the dye coupled with 4-AA; However, 4-AA solution was more stable than MBTH solution. The substrate is enzymatically oxidized by its oxidase to produce hydrogen peroxide. The concentration of hydrogen peroxide corresponds to the substrate concentration. Therefore, the amount of the substrate can be determined by the color development of the oxidative coupling reaction. Glucose, alcohol, acyl coenzyme A and cholesterol can be used to detect those substrates coupled with the novel Trinder's reagent and 4-AA. There are 10 new Trinder's reagents. Among the new trinder's reagents, toos is the most commonly used. However, for specific substrates, it is necessary to test different kinds of novel trinder's reagents to develop an optimal detection system

Storage: RT, protected from light. Shelf life: 5 years