Rnasin Inhibitor

English name：Rnasin Inhibitor
Other name: RNase inhibitor

Grade：BR
Molecular weight：51000
Description: RNasin is a specific RNase inhibitor extracted from human placenta. It is a protein with a molecular weight of 51000 daltons and an isoelectric It can specifically combine with RNase to form a complex with a non covalent bond with an inhibition constant (KI) of 300 and inactivate RNase. The activity was maintained when the buffer was 0-0.5m NaCl and pH 5-8, and the activity was the highest at pH 7.8.Through strict quality inspection, the product is electrophoretically pure, free of RNase and nickel pollution, and the specific activity is 100000 units / mg protein.

Characteristics of RNA enzyme inhibitors：
1. Inhibition of general eukaryotic RNases, including RNase A, B, C and human placental RNase;

2. Do not inhibit RNase H, S1 nuclease, SP6, T7 and T3 RNA polymerase, AMV or m-mlv reverse transcription;

Enzyme, Taq DNA polymerase, RNase T1;

3. The active pH range is wide, but 1mm DTT is required to maintain its activity.

Concentration：40u/ul
RNA enzyme inhibitors unit definition：The amount of enzyme required to inhibit 50% of 5ng RNase A activity is one unit

Storage buffer：20mM HEPES-KOH pH7.6, 50mM KCl, 8mM DTT, 50%g lycerol

Selective inhibition of nuclease by RNA enzyme inhibitors：Inhibit  RNase A、RNase B、RNase C；RNase T1, S1 nuclease and RNase from Aspergillus were not inhibited.

Physicochemical properties of RNase inhibitors:

1. Activity: Inhibit RNase activity by non covalent binding with enzyme molecules

2. Inhibition type: Competitive inhibition

3. Binding constant: ki = 4.4 × 1014. Binding ratio with RNaseA: 1:1 

5. Free sulfhydryl: 30

Properties: Suspension

Purpose: Biochemical research. Ribonuclease inhibitors prevent ribonuclease from degrading fragile RNA during laboratory operations by tightly binding with ribonuclease. Used in places with potential RNase contamination. Protect mRNA in cDNA synthesis.In vitro transcription system and translation system to protect mRNA. It is used to increase the activity and yield of polyribosomes. Enhance virus replication in vitro. Promote RNA translation in homologous systems. RNase free antibodies were prepared. Improve the yield of riboprobe (R) system RNA synthesis reaction.