Luminol/3-Aminophthalhydrazide, 5-Amino-2,3-dihydro-1,4-phthalazinedione

English Name: luminol; 3-Aminophthalhydrazide, 5-Amino-2,3-dihydro-1,4-phthalazinedione

Other names: 3-amino Phthaloyl hydrazine; Luminescent ammonia; 3-aminobenzoyl hydrazine; Cold light agent; 3-aminobenzoyl hydrazine; 5-amino-2,3-dihydro-1,4-diazaphthalene Dione; 3-aminophthalic anhydride

CAS No.: 521-31-3

C8H7N3O2=177.16

Grade: br

Dye content: ≥ 98.0%

Loss on drying: ≤ 0.5%

Luminous sensitivity test: acceptable

Alkali solution test: acceptable

Melting point: 140 ~ 143 ℃

Physicochemical properties  : white or light yellow crystal. It is easily soluble in alkali liquor, soluble in dilute acid, insoluble in water and organic solvents, and insoluble in alcohol. Neutral or light acidic solutions show strong bright blue fluorescence when exposed to ultraviolet light. The melting point is 319 ~ 320 ℃. Luminol is one of the oldest and most commonly used reagents, which can be oxidized by peroxides under alkaline conditions and emit light at the same time. The redox reaction between luminol and peroxides requires a catalyst, which is generally multivalent metal ions, peroxidases such as iron, spicy root peroxidase, etc. this method is commonly used to detect the content of peroxides, heavy metals, peroxidases, and the derived free radicals Carry out toxicological analysis and analytical methods based on peroxidase and glucose oxidase. Under normal circumstances, luminol and hydrogen peroxide react very quickly in the presence of some catalysts. The most commonly used catalyst is metal ions. In a large concentration range, the concentration of metal ions is proportional to the luminous intensity, so the chemiluminescence analysis of some metal ions can be carried out. Using this reaction, those organic compounds containing metal ions can be analyzed to achieve high sensitivity. The second is to use the inhibition of organic compounds on luminol chemiluminescence reaction to determine the organic compounds that have quenching effect on chemiluminescence reaction. The third is the indirect determination of inorganic or organic compounds through coupling reaction

Purpose: biochemical research. Luminescence test: EmMax 440 nm (chemiluminescence; 60 mm K2S2O8, 100 mm K2CO3, pH 11.5; after adding H2O2), chemiluminescence reagents and indicators are commonly used in chemiluminescence analysis, such as metal cations, blood immunity, etc

Save: RT, away from light