α-Chymotrypsin(bovine)；Chymotrypsin(bovine)；TLCK-Chymotrypsin CAS 9004-07-3
Item No.： E0002
Store at： -20℃
English Name: α- Chymotrypsin(bovine)； Chymotrypsin(bovine)； TLCK-Chymotrypsin
Other names: α- Chymotrypsin; α- Chymotrypsin; Trypsin; Coagulation trypsin
Grade: USP level
Molecular weight: 25kDa
Vitality: ≥ 1500usp units / mg
Definition of activity: at 25 ℃, pH 7.0, the amount of enzyme that reduces the optical density value at 237nm by 0.001 per minute is 1atee unit (U)
Loss on drying: ≤ 5.0%
Burning residue: ≤ 2.5%
Physicochemical properties : white or nearly white crystalline or amorphous powder. No smell. Soluble in water and dilute buffer solution. The optimum pH is 7.5 ~ 8.5. Isoelectric point (PI) 8.3. LD50 (rat, intravenous) 84mg / kg. It is a proteolytic enzyme, which can preferentially hydrolyze the peptide bond formed by the carboxyl group of L-tyrosine and L-phenylalanine. Chymotrypsin activity can be inhibited by heavy metals and natural trypsin inhibitors in varying degrees.
Purpose: biochemical research. Protein decomposition and digestion. Proteolytic enzyme, used for protein hydrolysis in the absence of exogenous trypsin activity. It has the function of decomposing peptide bonds. The carboxyl end of hydrolyzed peptide bonds has aromatic or long hydrophobic chains (Tyr, Trp, Phe, met).
