Solid green FCF / fast green FCF / fast green / food color green No. 3 / food green 3

English Name: fast green FCF; Food green 3； C.I. 42053

Other names: Solid green; Fast green; Firm green; Edible pigment green No. 3; Food green 3

CAS No.: 2353-45-9

C37H34N2Na2O10S3=808.85

Grade: high purity grade

Effective spectral content: ≥ 90.0%

Loss on drying: ≤ 8.0%

Em (622nm, 50% Methanol)：>125000

Physicochemical properties: dark green powder or maroon particles with metallic luster. It is hygroscopic. It is soluble in water, soluble in ethanol, dark orange in concentrated sulfuric acid, dark green after dilution, orange in concentrated hydrochloric acid or concentrated nitric acid, and bright blue in 10% sodium hydroxide solution. Melting point 290 ℃ (decomposition). The maximum absorption wavelength is 628nm. LD50 (rat, oral) > 2G / kg

Purpose: biochemical research. Staining of plant histology and cytology. It is an alkaline dye used for protein staining in IEF, page and SDS-PAGE. Fast green staining covers a wider range of protein concentrations than bright blue R and is linear. After electrophoresis, the protein was fixed on the gel and stained more sensitively. The gel was stained with 0.1% fast green dissolved in 30% (V / V) ethanol, 10% (V / V) acetic acid or 7% acetic acid for 2 hours, and then stained with 30% (V / V) ethanol, 10% (V / V) acetic acid or 7% acetic acid The dye gel can be viewed at 625 nm. The detection sensitivity is 30% higher than that of brilliant blue R. In addition, fast green is also used for sulfite determination, producing bacterial culture medium to identify E. coli and other lactose fermentation bacteria, and culturing and isolating typhoid bacteria in feces

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