Fluoramine / flurane / fluorescaine

English name：Fluorescamine；Fluram；4-Phenylspiro[furan-2(3H),1′(3′H)-isobenzofuran]-3,3′-dione；4-Phenylspiro-[(furan-2(3H),1′-phthalan]-3,3′-dione ,Ro-20-7234

Other names: fluorescein; Florand; Fluralam; 4-phenylspiro [furan-2 (3H), 1-dihydroisobenzofuran] - 3,3 '- Dione; 4-phenylspiro - [furan-2 (3H), 1-piperidine] - 3,3 ′ - Dione

CAS No.: 38183-12-9

C17H10O4=278.26

Grade: biochemika

Content: ≥ 99.0% (UV)

fluorescence： λ ex 390 nm;  λ em 475 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine)

Melting point: 153 ～ 157 ℃

Solubility: acetonitrile: soluble; ethanol: soluble

Physicochemical properties: off white or light yellow powder, sensitive to humidity. It does not fluoresce with the hydrolysate, but generates similar compounds with strong fluorescence with the compounds containing primary amino groups (including proteins, peptides, amino acids and other various polypeptides). The maximum excitation wavelength of the product is 390nm and the maximum emission wavelength is 475nm.

Purpose: biochemical research. The non fluorescent reagent can form a stable, high fluorescence and low background complex with the primary amino groups of amino acids and proteins under mild conditions, and is used for fluorescence detection of amino acids, proteins and proteolytic enzymes. Also used in protein sequencing analysis.

Saving: RT